Research Journal of Pharmacognosy and Phytochemistry

Gottumukkala Krishna Mohan ¹, Malavika Yadav ¹, M. Sandhya Rani ¹, Kalakotla Shanker ¹

Affiliations
1 Centre for Pharmaceutical Sciences, IST, Jawaharlal Nehru Technological University, Hyderabad-500085, Telangana, IN

Abstract

The Antimitotic Activity of Methanolic extract of Borassus flabellifer L. (Arecaceae) seed coat (soft outer shell) was studied by using Seed Germination Assay and Allium cepa Root Tip Assay. The preliminary phytochemical screening of methanolic seed coat extract of Borassus flabellifer revealed that presence of carbohydrates, reducing sugars, coumarins, triterpenes, tannins and phenolic compounds. In Seed Germination Assay, the methanolic seed coat extract of Borassus flabellifer has significantly increased the percentage of inhibition after 24hrs, 48hrs and 72hrs treatment which was comparable with percentage of inhibition of methotrexate. At dose 50mg/ml (p<0.0001) extract has shown the inhibition of seed germination as significant as the standard drug methotrexate (0.1mg/ml)(p<0.0001). In Allium cepa ischolar_main tip assay, the percentage of mitotic index was decreased significantly after 1, 3 and 6 hrs of drug treatment which was comparable with the percentage mitotic index of methotrexate. At dose 150mg/ml (p<0.0001) extract has shown antimitotic activity i.e., mitotic index as significant as of methotrexate of 0.1mg/ml (p<0.0001). Thus, the Methanolic seed coat extract of Borassus flabellifer is recommended for the treatment of cancer. Further in-vivo parameters need to be done for mechanistic evaluation of drug.

Keywords

Antimitotic Activity, Borassus flabellifer, Seed Germination Assay, Allium cepa Root Tip Assay, Methotrexate.

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E. Swapna ¹, G. Krishna Mohan ¹, P. Nagaveni ¹, Kalakotla Shanker ¹

Affiliations
1 Centre for Pharmaceutical Sciences, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500 085, Telangana, IN

Abstract

Ayurvedic medicine or Ayurveda is one of the world's ancient medical systems. It originated in India and has evolved here over thousands of years. The term "Ayurveda" combines the Sanskrit words ayur (life) and veda (science or knowledge) which means "The science of life". Ayurveda was the first holistic system of diagnosis, treatment integrating nutrition, hygiene, rejuvenation and herbal medicine. Balarishta is a poly herbal hydro alcoholic preparation and is used to cure gastric problems as restorative and as prime tool for the treatment of Arthritis, Rheumatism and all other auto immune disorders. In present study the Balarishta formulations (B1, B2, and B3) were screened for the Antioxidant activity by using Reducing Power Assay, Hydrogen Peroxide Scavenging Assay, Nitric Oxide Scavenging Assay methods. Ascorbic acid was used as standard Antioxidant drug. Current study demonstrates that the obtained results of Balarishta formulation was showed the good Antioxidant activity near to compared with standard drug.

Keywords

Ayurveda, Balarishta, Antioxidant Activity, Hydrogen Peroxide Scavenging Assay, Nitric Oxide Scavenging Assay, Ascorbic Acid.

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